Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow thi...Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.展开更多
目的根据目前口腔综合治疗台水路(Dental Unit Waterlines,DUWLs)的微生物污染以及消毒方法的现状,开发一种外置式的程序控制自动DUWLs消毒装置,解决目前医院在用DUWLs的自动消毒问题,以提升临床口腔用水的安全性,保障患者安全,同时减...目的根据目前口腔综合治疗台水路(Dental Unit Waterlines,DUWLs)的微生物污染以及消毒方法的现状,开发一种外置式的程序控制自动DUWLs消毒装置,解决目前医院在用DUWLs的自动消毒问题,以提升临床口腔用水的安全性,保障患者安全,同时减轻医护人员工作量。方法不改变现有口腔综合治疗台水、气及电路结构,通过程序控制实现消毒流程和各种消毒液的配比自动化。同时,利用口腔综合治疗台的气源将消毒液注入DUWLs内各管路中,以此对DUWLs各狭小管路内消毒及细菌的滋长进行抑制。通过采集口腔综合治疗台消毒前后的DUWLs水样样本,对细菌总数的变化进行比较。结果在使用自动DUWLs消毒装置对DUWLs进行消毒后,通过高速手机及漱口水水样样本的结果可看出DUWLs中的细菌菌落总数相对于消毒前明显减少,达到供水要求。消毒后与消毒前的数据差异具有统计学意义(P<0.05)。结论使用DUWLs自动消毒装置一方面可有效减少DUWLs的细菌污染,为患者提供安全地临床用水;另一方面比传统手工消毒更加便捷、全面。展开更多
目的:探讨脐带血细菌污染阳性率及不同设备在脐带血培养中的异同。方法:统计2015~2017年山东省脐带血造血干细胞库的血培养情况,比较培养天数对细菌检出率的影响,同时比对两种设备的细菌阳性率和使用情况。结果:2015年、2016年、2017年...目的:探讨脐带血细菌污染阳性率及不同设备在脐带血培养中的异同。方法:统计2015~2017年山东省脐带血造血干细胞库的血培养情况,比较培养天数对细菌检出率的影响,同时比对两种设备的细菌阳性率和使用情况。结果:2015年、2016年、2017年的细菌阳性率分别为2.69%、2.33%、2.05%。培养5天能检测出99%以上的细菌,采集环节是脐带血污染的主要部分,约占90%。两种血培仪设备(法国梅里埃BacT/A LERT 3D 240和美国BD BA CTEC^TM FX)的细菌阳性率基本相同。结论:脐带血细菌污染阳性率逐年降低,全自动化血培养5天符合检测要求,加强临床采血的无菌操作,可降低脐带血污染的几率。两种全自动化血培仪的细菌阳性率无显著差异。展开更多
文摘Portable diagnostic devices are a viable and low-cost alternative for the detection of pathogens, since they reduce the time of analysis of results availability. Ease of sample collection and quick diagnosis allow this new input to be applied in the diagnosis of the main contaminating microorganisms present in the water. Laboratory tests evaluated the technical viability of the diagnostic device, using commercial strains which were inoculated and optimized in the devices and their growth compared to the conventional method in Petri dishes. Samples of 100 μL bacterial suspension were tested and compared with the traditional sample inoculation method. The device viability was determined by detecting characteristic bacterial colonies in a specific culture medium through the colorimetric development of the obtained colonies. The feasibility assessments allow us to affirm that the device enables both qualitative and quantitative detection of the target bacteria present in liquid samples, and is promising to be applied to assess the quality of water, food and environmental surfaces.
文摘目的根据目前口腔综合治疗台水路(Dental Unit Waterlines,DUWLs)的微生物污染以及消毒方法的现状,开发一种外置式的程序控制自动DUWLs消毒装置,解决目前医院在用DUWLs的自动消毒问题,以提升临床口腔用水的安全性,保障患者安全,同时减轻医护人员工作量。方法不改变现有口腔综合治疗台水、气及电路结构,通过程序控制实现消毒流程和各种消毒液的配比自动化。同时,利用口腔综合治疗台的气源将消毒液注入DUWLs内各管路中,以此对DUWLs各狭小管路内消毒及细菌的滋长进行抑制。通过采集口腔综合治疗台消毒前后的DUWLs水样样本,对细菌总数的变化进行比较。结果在使用自动DUWLs消毒装置对DUWLs进行消毒后,通过高速手机及漱口水水样样本的结果可看出DUWLs中的细菌菌落总数相对于消毒前明显减少,达到供水要求。消毒后与消毒前的数据差异具有统计学意义(P<0.05)。结论使用DUWLs自动消毒装置一方面可有效减少DUWLs的细菌污染,为患者提供安全地临床用水;另一方面比传统手工消毒更加便捷、全面。
文摘目的:探讨脐带血细菌污染阳性率及不同设备在脐带血培养中的异同。方法:统计2015~2017年山东省脐带血造血干细胞库的血培养情况,比较培养天数对细菌检出率的影响,同时比对两种设备的细菌阳性率和使用情况。结果:2015年、2016年、2017年的细菌阳性率分别为2.69%、2.33%、2.05%。培养5天能检测出99%以上的细菌,采集环节是脐带血污染的主要部分,约占90%。两种血培仪设备(法国梅里埃BacT/A LERT 3D 240和美国BD BA CTEC^TM FX)的细菌阳性率基本相同。结论:脐带血细菌污染阳性率逐年降低,全自动化血培养5天符合检测要求,加强临床采血的无菌操作,可降低脐带血污染的几率。两种全自动化血培仪的细菌阳性率无显著差异。